Understanding microorganisms: DNA extraction explained

19 Jun 2022

Microorganisms are the foundation of the biosphere and perform most biochemical processes on the planet, yet many remain unidentified. The diverse and complex communities of these microbes each occupy a unique niche in the ecosystem. Gaining a comprehensive knowledge of these microorganisms is essential, particularly when trying to improve our understanding of the environment, the food we consume, the fuel we use, and the gut microbiota.

This application eBook presents an expert guide on how to effectively extract DNA to better investigate microorganisms, details the complexity of freshwater environments, explores antibiotic resistance genes (ARGs) in wastewater treatment plants, and illustrates how high-throughput sequencing can help accurately detect helminth infections.

This eBook will also cover:

  • The analysis of fermented foods
  • How to prevent biases in microbiome extraction and sequencing
  • The latest technologies designed to help accelerate your research and achieve optimum DNA extraction

FastPrep-24™ 5G

MP Biomedicals

The FastPrep-24™ 5G Instrument is quite simply the most advanced sample preparation system ever!   The only high-speed benchtop homogenizer featuring microprocessor-controlled, Hi-Def Touch Screen Programming makes the 5G the ultimate in ease, speed and performance for sample preparation applications where it is necessary to grind, lyse, or homogenize difficult and routine samples, allowing easy and reproducible isolation of stable RNA, active proteins and full-length genomic DNA. Applications include but are not limited to: biological tissues, fluids and excretions cell cultures environmental sample other inorganic solid matrices. The FastPrep-24™ 5G Instrument uses a unique, optimized motion to disrupt cells through the multidirectional, simultaneous beating of specialized Lysing Matrix beads on the sample material. Samples and buffers are added to Lysing Matrix Tubes containing specialized Lysing Matrix beads, supplied ready-to-use, certified nuclease-free and in a variety of sample type specific compositions. The sample tubes remain securely sealed during the processing and the single use design eliminates cross-contamination. Program parameters are easily set using the touch screen user interface, or choose from many Recommended Programs stored on the 5G's onboard computer. With a simple touch of the "run" button, in less than a minute your samples are completely lysed, super fast, every time! The highest power settings available ensure that DNA, RNA and proteins, even from difficult samples, are quickly liberated into the protective buffer, thus preserving their biological function. FastPrep-24™ 5G Exclusive Features Powerful - Highest speed range available, ideal for a wide range of applications and protects your sample Optimized - Cycle and pause programmable for true walk-away operation Convenient - >70 Recommended Programs for easy optimizations Intuitive - Interactive user-friendly interface and touchscreen Flexible - Optional sample holders allow processing from 2 - 50 mL tubes, under ambient or cryogenic conditions Smart - Integrated USB Port allows for software updates to be installed, and run history data to be downloaded to a laptop Safe - Electromechanical dome latch automatically secures processing chamber, with a simple finger touch Complete - Lysing Matrix Tubes and Purification Kits from One Source

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FastDNA SPIN Kit™

MP Biomedicals

  Rapid Method of Isolating Pure Genomic DNA from a Wide Variety of Sources!   Isolate PCR-ready DNA from a variety of sample types No hazardous organic reagents are required The FastDNA™ SPIN Kit quickly and efficiently isolates genomic DNA from almost any sample (plant and animal tissues, cultured cells, bacteria, yeast, fungi, insects, etc). Up to 200 mg of tissue or cells are processed by the FastPrep-24™ 5G, FastPrep FP120™, FastPrep-96™ or SuperFastPrep-1™ with the Lysing Matrix A tubes. The kit includes 3 different chaotropic buffers for the homogenization of a wide variety of sample types and the released DNA is purified by a silica-based spin filter method. Purified DNA is ready for enzyme digestion, electrophoresis, PCR and any other desired application.

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Water PurificationThere are a number of water purification methods available to remove contamination from laboratory water. These systems include demineralization, reverse osmosis, filtration and ultrafiltration. A combination of methods is sometimes necessary to achieve purified water suitable for use in experiments.Waste WaterFood TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.Biofilm