Transfection efficiency of GFP- and mcherry-expressing HeLas evaluated on a microplate reader

Cells are transfected with exogenous DNA to study the regulation of gene and protein expression. To monitor transfection efficiency, a reporter gene is often attached to the gene of interest to monitor its insertion into the cell’s genome. Fluorescent proteins like Green Fluorescent Protein (GFP) are often used as such reporters during transfection efficiency experiments. In this application note, find out how BMG LABTECH readers can reliably detect cells expressing a fluorescent marker down to ~600 cells/well in a 96- well plate.