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Tips for optimizing your immunofluorescence staining
11 Mar 2021Immunofluorescence (IF) is a powerful tool used to detect and identify the subcellular distribution and movement of proteins and other molecules within cells and tissues. In this application note, Biorbyt discusses when to use IF and outlines the differences between IF, immunohistochemistry (IHC), and immunocytochemistry (ICC).
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CD133 antibody
BiorbytRabbit polyclonal antibody to CD133; which is also known as prominin or AC133. CD133 is a marker frequently found on multipotent progenitor cells; including immature hematopoietic stem and progenitor cells. The protein has been extensively used as a stem cell marker for normal and cancerous tissues.
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.ImmunofluorescenceImmunohistochemistryImmunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples by using antibodies that bind to target antigens. IHC is widely applied in pathology, immunology, hematology and cancer diagnostics. Explore IHC tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.