ResourceLife Sciences
The ultimate guide to building, managing, and analyzing your plasmid library
3 Aug 2023Plasmids are small, circular, double-stranded DNA molecules that can exist independently of chromosomal DNA in various host model organisms. Since their discovery and application in the life sciences using recombinant DNA technology, they’ve become an indispensable part of any R&D lab performing genetic engineering, designing and expressing recombinant proteins, developing new vaccines or therapies, and studying gene function and regulation. In this whitepaper, eLabNext introduces some of the common plasmids used in the life science space and provides some best practices for building, maintaining, managing, and storing a plasmid library.
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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.