The quantification challenges of chromatin
8 Apr 2025Chromatin fragments from host cell DNA contaminate protein-based therapeutics, viral vectors, and vaccines. Regulatory guidelines mandate stringent DNA removal, but chromatin’s protein-bound structure hinders detection by intercalating dyes like PicoGreen, leading to underestimation.
Discover how to enhance detection sensitivity and ensure more accurate quantification with an alternative approach that performs DNA fragmentation prior to measurement. Learn how this minimizes the risk of undetected carryover in downstream processing and highlights the importance of nuclease treatment in host cell–based bioprocessing.
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DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.Host Cell Proteins