The CLARIOstar with ACU exposes cells to ischemia-reperfusion conditions and monitors their oxygenation

The lack of oxygen supply is associated with a number of life-threatening diseases such as stroke, myocardial infarction or renal failure whereby cells are temporarily deprived of O₂ and nutrient (ischemia). Significant cell damage can also occur during the reperfusion phase through oxidative stress and inflammatory responses. Investigating these pathologies in vitro requires an experimental set-up capable of rapid deoxygenation, rapid reperfusion, and parallel monitoring of critical biological parameters including cellular oxygenation and ROS. The ischemia-reperfusion model presented here uses the CLARIOstar^® microplate reader by BMG LABTECH with software-controlled programmable O₂ and CO₂ regulation in combination with MitoXpress^®-Intra, (Luxcel Biosciences) which enables real-time monitoring of cellular oxygenation.