The central role of immune cell metabolism in building next-generation cell therapies
3 Feb 2023As sentinels, poised to rapidly respond to a host of triggers from foreign antigens to cancer cells, immune cells can modulate their function in a precise and controlled manner. In this interactive eBook, Agilent Technologies provides an introduction to the real-time measurement of immune cell bioenergetics using the Agilent Seahorse XF Analyzer. It also references recently published examples to illustrate how leading investigators and organizations in the field of immunotherapy have applied these tools to address the challenges of cell engineering and testing cell constructs, as well as monitoring efficacy through cell therapy manufacturing.
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Seahorse XF Cell Mito Stress Test Kit
Agilent TechnologiesThe Agilent Seahorse Cell Mito Stress Test kit is the widely recognized, well accepted standard assay for assessing mitochondrial function. Multiple parameters are obtained in this one assay including, basal respiration, ATP-linked respiration, maximal and reserve capacities, and non-mitochondrial respiration. The assay provides insights into the mechanism of mitochondrial dysfunction and allows users to investigate functional differences among cell types, drug candidates, and genetic or biochemical interventions. Each kit contains the material sufficient for six full plate tests and is available in two packaging sizes. The XF kit is for use with the Agilent Seahorse XF Pro, XF96/XFe96 and XFe24 Analyzers. The XFp kit is for use with the Agilent Seahorse XF HS Mini and XFp Analyzers.