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Targeted Analyses of Secondary Metabolites in Herbs, Spices, and Beverages Using a Novel Spectro-Electro Array Platform

Thermo Fisher Scientific
29 Apr 2014

This application note describes a method using a gradient HPLC spectro-electro array platform to resolve and quantify specific polyphenols in crude extracts of a variety of natural products, supplements (ginseng, black cohosh, St. John’s wort, and ginkgo), beverages (black tea, green tea, wine, beer, whisky, and bourbon), culinary herbs (oregano, rosemary, sage, and thyme), and spices (cloves and nutmeg).

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Thermo Scientific™ UltiMate™ 3000 UHPLC system

Thermo Fisher Scientific

  The Thermo Scientific™ UltiMate™ 3000 HPLC system offers UHPLC compatibility across all modules, ensuring maximum performance. Covering flows from 20 nL/min to 10 mL/min and offering a wide range of pumping, sampling, and detection modules, the UltiMate 3000 series provides solutions for all your chromatography needs. UltiMate 3000 HPLC system Features & Benefits:   UHPLC design for all nano, standard analytical, and RSLC systems. 620 bar maximum pressure for basic and standard analytical systems—setting a new benchmark in HPLC. x2 Dual Systems form a unique platform for routine analysis, increased productivity solutions, and advanced chromatographic techniques. Systems are controlled using Thermo Scientific™ Chromeleon™ Chromatography Data System software—providing Intelligent Functionality and Operational Simplicity™. Systems are offered with Thermo Scientific™ Viper™ and Thermo Scientific™ nanoViper™ fitting systems—fingertight, zero-dead-volume connections even at UHPLC pressures.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.UV-Visible SpectroscopyUltraviolet-visible (UV-Vis) spectrophotometers are used to measure the interaction of UV and visible light with a sample, including transmission, reflectance & absorbance. The two major instrument classes are single-beam or double-beam spectrophotometers. More specialized equipment includes colorimeters, spectroradiometers and refractometers. Portable and microvolume spectrophotometers are also available. For the modular spectroscopy lab, explore a range of light sources for combination with a spectrograph/spectrometer and optics. Find the best UV-Vis spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.PolyphenolsBeverage AnalysisBeverage analysis involves testing and evaluating the composition, quality, and safety of drinks, from alcoholic beverages to soft drinks. Techniques like chromatography and spectroscopy are commonly used to analyze flavors, nutrients, contaminants, and additives. Browse our peer-reviewed product directory to find the best beverage analysis tools, compare products, check reviews, and get pricing directly from manufacturers.Food FraudFood adulteration refers to food when it has been has been altered unlawfully with the addition of another lower commodity food product to increase quantity. The 2013 European horsemeat scandal saw the adulteration of beef products with the addition of horse meat.

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