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TARGATT large knock-in technology

Applied StemCell
1 Sept 2025

While CRISPR has transformed gene editing, it presents limitations for applications requiring large DNA insertions in mammalian cells, including low knock-in efficiency for fragments over 3–5 kb and risks associated with double-strand breaks.

Explore how TARGATT™ gene knock-in technology from Applied StemCell addresses these challenges through a precise, efficient, and scalable approach. Utilizing a three-component system, TARGATT integrase, a defined H11 safe harbor site, and a donor plasmid enables targeted, single-copy insertion of DNA fragments up to 20 kb. Its unidirectional, site-specific integration ensures stable expression and genomic integrity, making TARGATT a robust solution for complex gene engineering and therapeutic development.

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Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.Cell Line Development

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TARGATT large knock-in technology