T-Cell Adhesion to ICAM-1

1 Oct 2012

This application note investigates the adhesion of T-cells to ICAM-1 when treated with a range of statins (pravastatin, fluvastatin, mevastatin, lovastatin and simvastatin) under physiological flow. The method presented uses Cellix’s biochips and Mirus pumping system.

Vena8 Endothelial+ Biochip: microfluidic flow chamber

Cellix Ltd

The Vena8 Endothelial+™ biochip is ideal for studying cell-cell interaction. Endothelial cells are easily seeded (via standard pipette) and cultured inside the microcapillaries of the biochip. As cells flow through the microcapillary of the Vena8 Endothelial+™ biochip, the cell surface receptors may interact with the endothelial cell monolayer which coats the microcapillary walls. Ideal for use with phase contrast imaging and fluorescence imaging to study cell-cell interactions under conditions mimicking physiological flow.

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Vena8 Fluoro+ Biochip: microfluidic flow chamber

Cellix Ltd

The Vena8 Fluoro+™ biochip is ideal for studying cell receptor-ligand interaction and is compatible with confocal microscopy. As cells flow through the microcapillary of the Vena8 Fluoro+™ biochip, the cell surface receptors may interact with adhesion molecules or ligands which coat the microcapillary walls. Ideal for use with fluorescence immunostaining, confocal microscopy and for applications such as thrombosis.

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Cell Adhesion AssaysCell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for extracellular matrix adhesion, cell migration and sensitivity to inhibitors. Find the best cell adhesion assay equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Lab-on-a-ChipLab-on-a-chip (LOC) technology uses microfluidic chips, such as microarrays, to perform biochemical assays on a small scale. Benefits of lab-on-a-chip include the small volume of sample required as well as the speed of analysis. In selecting lab-on-a-chip devices consider the assay, cost, reliability, sensitivity and signal to noise ratio.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.PumpsLaboratory pumps are devices used to move liquids and gases by vacuum or positive displacement. The pressures/vacuums generated are determined by the type of pump used. High vacuum pumps include diffusion, ion, roots and turbopumps; for primary/medium vacuum applications see rotary vane pumps, scroll & diaphragm pumps. For the positive displacement of liquids explore a range of syringe and peristaltic pumps, as well as fluid aspirators. Discover the best pumps in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.SepsisSepsis is a life-threatening response to infection causing organ dysfunction. Research focuses on early detection, biomarkers, and therapies to reduce mortality. Explore tools for sepsis diagnostics and analysis in our peer-reviewed product directory, including detection kits and biomarkers.StatinsT Cells
T-Cell Adhesion to ICAM-1