Streamlining NGS Quality Management Using High-Throughput Fluorometric DNA/RNA Quantification

This application note looks at the quantification of nucleic acids for next-generation sequencing (NGS) applications using the FLUOstar Omega microplate reader. A fluorescent nucleic acid quantification was performed in high-throughput screening format and the results were integrated into the automatic workflow of NGS sample preparation. Here it was shown that combining Qubit assays with the FLUOstar Omega multi-mode microplate reader is ideally suited to measure a wide range of nucleic acids and improves compared to the single tube fluorometer on sensitivity, precision and speed. Especially large laboratories that perform automated high throughput sample preparation will profit from this technology.