Real-Time Quantitative PCR Performance Characterization with IntelliQube

20 May 2019

In this application note, LGC Biosearch Technologies highlights a series of experiments that demonstrate the robustness of the IntelliQube system. The product promises to combine quantitative real-time PCR, liquid handling, and reagent cost savings into one convenient and easy-to-use laboratory instrument.

IntelliQube™ integrated PCR workflow

LGC Diagnostics and Genomics - Rapid Genomics Genotyping Solutions

Fully Automated PCR Setup, Amplification and Analysis System. The IntelliQube is the first fully automated and high-throughput instrument featuring seamless integration of liquid handling, thermal cycling, detection, and data analysis. The IntelliQube supports quantitative real-time PCR, end-point PCR, and isothermal chemistries, allowing you to realize the benefits of inline automation enabled by the innovative Array Tape™ consumable. IntelliQube leverages best-in-class multi-functional dispensing for high speed liquid handling in 384- or 768-well Array Tape™. Precise sample dispensing into Array Tape is handled by an interchangeable CyBi® FeliX 96- or 384-channel pipetting head. The IntelliQube supports inline amplification with a Peltier thermal cycling block designed specifically for Array Tape™. Due to a unique block design and surface geometry, both 384- and 768-well Array Tape are supported, without requiring a block change. The IntelliQube features an advanced optical system optimized for inline fluorescence detection in Array Tape. Filtered LEDs are optimized for a variety of commonly used fluorophores excited within the range of 480-620 nm. A high-resolution CCD camera supports detection of fluorescence intensity within 5 detection channels for dyes emitting within the range of 510-705 nm. Multiplex data capture for all 5 optical channels can occur in as little as 10 seconds. Benefits: Walk-away operation Increased efficiency of lab personnel Reduced chemistry costs Increased throughput Reduced consumable costs

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.
Real-Time Quantitative PCR Performance Characterization with IntelliQube