Real-time detection and modulation of human T cell activation

Agilent Seahorse XF technology delivers real-time insights into cellular energy metabolism, and is relevant to the study of T cell activation and function. This utility is now extended by the Agilent Seahorse XF Hu T Cell Activation Assay, which provides a rapid and standardized method to measure early activation-associated metabolic responses in human T cells, offering important insights into both the early dynamics of activation and its broader metabolic underpinning. The XF Hu T Cell Activation Assay workflow incorporates a soluble CD3/CD28 activator, precoated poly-D-lysine (PDL) XF Cell Culture Microplates, and Agilent Seahorse Analytics, an intuitive, cloud-based data analysis tool.