Seahorse XFe24 Analyzers
XFe Extracellular Flux Analyzers simultaneously interrogate the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a microplate, in real-time.
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Promising results
Immunology
Immunometabolism was always neglected before Seahorse XFe Analyzer. I used Seahorse XFe96 Analyzer and applied it in the field of immunotoxicology and showed in my research articles how the study of Immunometabolism of cells in presence of nanoparticles gets affected. In the future, I expect in drug discovery and toxicology studies seahorse XFe Analyzer will be one of the key instruments. Publications Published 1) Dey, A.K., et al., Impact of Gold Nanoparticles on the Functions of Macrophages and Dendritic Cells. Cells, 2021. 10(1). 2) Dey, A.K., et al., Tuning the Immunostimulation Properties of Cationic Lipid Nanocarriers for Nucleic Acid Delivery. Frontiers in Immunology 12.
Review Date: 12 Feb 2023 | Agilent Technologies
XFe Extracellular Flux Analyzers simultaneously interrogate the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a microplate, in real-time.
The measurement of cellular bioenergetics on live cells enables time-resolved analysis and testing of multiple conditions per assay well. XF assays provide increased throughput and use less sample compared to conventional respirometry techniques. XFe Analyzers are available in two formats- 96-well for maximum throughput and flexibility, and 24-well for specialty samples such as islets and small model organisms.
Features:
- Live-Cell, Real-Time Assays
- Detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing
- Direct Measure of Energy Metabolism
- Validate results from protein/gene expression experiments with a functional assay
- Label-free platform
- No staining, analyte extraction, or destructive sample processing required
- Minimal Sample Required
- As few as 5000 cells/well (varies by cell type and instrument)
Rewiring the metabolism of CAR-T cells
Join Professor Mercedes Rincon, University of Colorado Anschutz School of Medicine, as she discusses the groundbreaking advancements in adoptive T cell therapy, particularly chimeric antigen receptor (CAR) T cell therapy. While CAR-T therapy has achieved impressive complete remission rates in 60–90% of patients with relapsed/refractory B-cell acute lymphoblastic leukemia (r/r-B-ALL), challenges such as treatment failures and relapses remain significant obstacles.
Metabolism plays a critical role in T cell function, shaping immune responses. While glycolysis is essential for T cell expansion, it can restrict the self-renewal capacity of CAR-T cells during manufacturing. Mitochondrial respiration is vital for their survival and effectiveness. Recent findings have highlighted MCJ, a protein that negatively regulates mitochondrial function in CD8 cells. Loss of MCJ enhances mitochondrial respiration, cytokine secretion, and anti-tumor activity. Targeting MCJ to boost mitochondrial metabolism presents a promising strategy to enhance the efficacy of CAR-T cells and advance adoptive T cell therapies.
Key learning objectives
- Understand the current landscape of adoptive T-cell therapy
- Explain the role of metabolism in T cell function, highlighting the significance of glycolysis and mitochondrial respiration in enhancing CAR-T cell efficacy
- Identify the potential of MCJ as a therapeutic target to improve mitochondrial metabolism, cytokine secretion, and overall effectiveness of CAR-T cell therapies
Who should attend?
- Researchers and scientists specializing in CAR T-cell therapy, immunotherapy development, cell metabolism, glycolysis, and mitochondrial respiration.
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
