ResourceSeparations

Rapid Separation of Anthocyanins in Cranberry and Bilberry Extracts Using a Core-Shell Particle Column

22 May 2016

This application note presents compares two HPLC methods for the qualitative analysis of bilberry extract, which is a complex sample with 15 anthocyanins and five anthocyanidins and the quantitative analysis of cranberry extract. The advantages of using a column that it is packed with solid core-porous shell particles, one of the newer developments in separation science, are described.

Thermo Scientific™ Acclaim™ 120 C18 Reversed-Phase LC Column

Thermo Fisher Scientific

High Performance Reversed-Phase Columns for the Separation of Small Molecules. The Acclaim® 120 columns are designed for high resolution reversed-phase separations. The very high surface coverage and very low metal content together result in columns with excellent efficiencies. These columns provide exceptional performance for a variety of applications in the pharmaceutical, chemical, environmental, and food separations areas.Acclaim 120 C18 columns have high surface coverage, resulting in high-capacity columns. They are stable between pH 2 and 8 and available in 3 and 5 μm particles sizes, and 4.6, 3.0, and 2.1 mm-diameters, with an average pore diameter of 120 Å. Acclaim 120 C18 is also available in 2.2µm RSLC format (see Acclaim Rapid Separation LC columns section for more details). All phases are LC/MS compatible because of the low silica bleed that results from the stable bonding procedure.Acclaim® 120 C18 Reversed-Phase LC Column Features: Ultrapure silica with high-density bonding for low silanol activity Very high efficiencies for maximum resolution Reproducible manufacturing practices for reproducible column-to-column performance LC/MS compatible Column Details: Particle Size: 3, 5 µm Pore Size: 120 Å Length: 50, 100, 150, 250 mm ID: 2.1, 3.0, 4.6 mm

(4)

Tags

UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Core-Shell TechnologyChromatography Method DevelopmentChromatography method development is the process of designing and optimizing chromatographic techniques to separate and analyze complex mixtures. This includes selecting appropriate stationary and mobile phases, determining flow rates, and optimizing detection methods. Successful method development is essential for high-quality results in fields like pharmaceuticals, food safety, and environmental analysis. Explore chromatography method development tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.FlavonoidsFood TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.
Rapid Separation of Anthocyanins in Cranberry and Bilberry Extracts Using a Core-Shell Particle Column