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QuEChERS Sample Preparation for the Determination of Polycyclic Hydrocarbons (PAH) in Shrimp Using LC with Fluorescence Detection
12 Feb 2013Polycyclic aromatic hydrocarbons (PAH) are toxic compounds commonly found in nature, and are constituents of coal and petroleum. Seven PAHs have been classified as probable human carcinogens by the US EPA. This application note describes a QuEChERS-based sample preparation protocol for the determination of PAH in shrimp. QuEChERS methodology is quicker, easier, cheaper and safer than prior methods of sample preparation.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.QuEChERSThe QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method is a streamlined approach that is used to extract multi-residue target compounds from food samples. It is most commonly used by analytical chemists studying pesticide residues in food.EPA MethodsEPA methods are procedures approved by the U.S. Environmental Protection Agency for monitoring and analyzing environmental pollutants, including air, water, and soil samples. These methods are critical in ensuring public health and environmental safety. Explore EPA methods and tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.SeafoodHydrocarbonPAHs
