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QuEChERS Sample Preparation for GC/MS Determination of Organophosphorous Pesticides in Beef

Waters
30 Nov 2012

Maximum reside limits (MRLs) are in place for many pesticides in animal products, therefore necessitating the determination of pesticide residues in these matrices. This application note demonstrates the suitability of QuEChERS as a methodology for determining organophosphorous pesticides in beef muscle tissue.This rapid method was developed for the analysis of pesticide residues in fruits and vegetables, prior to GC/MS or LC/MS and allows 30 samples to be prepared in 1 hour. Using the DisQuE pouch (QuEChERS) on ground beef with an 80% water content demonstrated that this method can effectively remove pesticides from beef with consistent recoveries ranging from 75% to 100%. The results indicate that QuEChERS extraction and subsequent dispersive SPE cleanup steps provide analysts with a fast preparation method using less toxic reagents, and with similar detection limits compared with other methods.

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DisQuE Dispersive Sample Preparation Kit

Waters

DisQuE Dispersive Sample Preparation is a well proven, high throughput sample preparation method for a wide array of pesticides in produce. The kit contains conveniently packaged vials with pre-measured sorbents and buffers used to provide extraction and clean-up for up to 100 samples. Each 50 mL DisQuE Extraction tube contains 6 grams of magnesium sulphate and 1.5 grams of sodium acetate. Each 2 mL DisQuE Clean-Up tube contains 150 mg of magnesium sulphate and 50 mg PSA (primary-secondary amine SPE sorbent). Detailed method instructions are included with the kit.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. GC-MS GC-MS (gas chromatography-mass spectrometry) instruments and equipment are used to separate, quantify and identify mixtures of small and volatile compounds, such as polycyclic aromatics, fatty acids and alcohols. Often used in drug detection, forensic investigation and environmental analysis for pesticides and contaminants, GC-MS is a powerful addition to your lab’s analytical capabilities. GC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzers can give greater sensitivity and resolution to your analysis. Find the best GC-MS instruments and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.QuEChERSThe QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method is a streamlined approach that is used to extract multi-residue target compounds from food samples. It is most commonly used by analytical chemists studying pesticide residues in food.PesticidesPesticides are used to protect crops from diseases, pests and unwanted plants. They are classed as persistent organic pollutants (POPs) and can be harmful to humans. The Environmental Protection Agency (EPA) currently regulates the use of pesticides in the USA. Food SafetyFood safety describes the prevention of food-borne illnesses. Contamination with a variety of pathogens can be prevented with adequate temperature control, hygiene and labeling. Food samples can be tested using GC/MS, HPLC, SPE, and qPCR to ensure their adherence to regulations.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.

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QuEChERS Sample Preparation for GC/MS Determination of Organophosphorous Pesticides in Beef