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Quantitative bioanalysis of oligonucleotides using a 6495D triple quadrupole LC/MS system combined with an automated SPE workstation

Agilent Technologies
11 Jun 2025

Discover the power of a high-throughput, automated workflow combining the Agilent 6495D triple quadrupole LC/MS system with Bravo-based solid-phase extraction (SPE). Designed for researchers and bioanalytical labs, it demonstrates how to achieve ultra-sensitive, reproducible quantification of therapeutic oligonucleotides in complex biological matrices, ideal for pharmacokinetic and toxicology studies.

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6495D triple quadrupole LC/MS (LC/TQ)

Agilent Technologies

If you are under pressure to deliver meaningful results, the Agilent 6495D LC/TQ provides sensitivity without compromising robustness.  Built-in instrument intelligence of the 6495D LC/TQ enhances throughput, and integrates comprehensive workflows to get your lab up and running fast, allowing even novice users to generate expert results. 

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.Bioanalysis

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Quantitative bioanalysis of oligonucleotides using a 6495D triple quadrupole LC/MS system combined with an automated SPE workstation