Qualitative and Quantitative Analysis of β-carotene Using UPC<sup>2</sup>

20 Jul 2014

As more stringent legislation for regulatory compliance of micronutrients in fortified food products and dietary supplements is being enacted or contemplated, there is an increasing demand for rapid and reliable analytical methods for the analysis and quantification of carotenoids in a variety of matrices. This application note describes the fast separations of three common carotenoids by UPC² in less than 2 minutes. A quantitative analysis of β-carotene dietary supplement capsules is also demonstrated.

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UHPLC and HPLCSupercritical Fluid ChromatographyUltraPerformance Convergence ChromatographyWorld Cup Dietary SupplementsFood Testing

ACQUITY UPC² System

Waters

Convergence Chromatography with the ACQUITY UPC² System Bring streamlined workflow, expanded selectivity, and an orthogonal technique to your laboratory. Convergence chromatography (CC) is a broad-based, complementary analytical platform that is taking its place alongside of LC and GC as one of the three essential separation technologies for modern laboratory analysis. This technique provides another, reliable choice as an analytical platform that is orthogonal to reversed-phase LC (RPLC), while significantly streamlining the entire analytical workflow. In convergence chromatography, the separation is achieved by manipulating the density and composition of a supercritical fluid-based mobile phase. Because of the very high diffusivity of the mobile phase, can be achieved. Additionally, the diversity of stationary phase and mobile phase (co-solvent) options provides access to the largest selectivity space available to any separation technique. The ACQUITY UPC² System is a holistically designed UltraPerformance Convergence Chromatography (UPC²) system that utilizes liquid CO2 as the primary mobile phase. The system leverages the chromatographic principles and selectivity of normal-phase chromatography while providing the ease-of-use of RPLC, enabling you to address routine and complex separations challenges. ACQUITY UPC² System: Discover what's been missing from your analytical toolbox With the ACQUITY UPC² System, scientists can now separate, analyze, and understand compounds that have challenged all other LC and GC technologies including: Hydrophobic and chiral compounds Lipids Thermally-labile samples Polymers It's also the perfect complement to MS due to its low solvent load and high resolution, narrow peaks and fast separations. ACQUITY UPC² System Features: Compressed carbon dioxide (CO2), the primary mobile phase, is less costly and less toxic than liquid mobile phases or carrier gases. Variable volume injection delivers 0.5 µL to 10 µL as well as the ability to match column and injection volume with minimal sample loss and need to change loops. Higher throughput and productivity allows you to analyze more samples per day with faster re-equilibration of columns and shorter cycle times, increasing laboratory productivity. Co-solvent and column switching provides flexibility in method development for faster solvent screening and faster column screening.

(18)

Qualitative and Quantitative Analysis of β-carotene Using UPC<sup>2</sup>

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2998 Photodiode Array (PDA) Detector

MassLynx MS Software

ACQUITY UPLC HSS T3 Columns for Polar Compound Retention

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