Preparation of Mouse Plasma Microsamples for LC-MS/MS Analysis Using the Echo Liquid Handler

8 Nov 2018

Serial microsampling of animals reduces the overall number of animals sampled and euthanized for toxicology studies. Traditional composite studies in mice can require up to 700 μL of blood for each time point in a pharmacokinetic study. A draw of 700 μL from a mouse would require the animal to be euthanized and often require the use of addition “satellite” animals to complete the study. Thus, in a 9-point time course analysis of mouse plasma, nine mice would be sacrificed. In response to programs like the EU 3Rs initiative to reduce the use of animals in research researchers have turned to microsampling as an alternative to composite studies. With advances in bioanalytical techniques drug levels can be determined from samples of 20 μL or less. This small sample volume or microsample enables reduces the dependency on satellite animals to complete a study. Additionally, microsampling is faster and less stressful than traditional composite studies.

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Echo® 500 Series Liquid Handlers
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Beckman Coulter Life Sciences
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Echo® 550 Liquid Handler
Beckman Coulter Life Sciences
Imagine preparing assay plates for siRNA screening, compound screening with biochemical and cell-based assays, PCR reactions and more all on a single liquid handling platform, with no tips and no cross-contamination. Labcyte has developed new acoustic droplet ejection (ADE) parameters that deliver built-in intelligence to enable users to transfer many different fluid types from a single instrument. All Echo systems come pre-calibrated so that you can switch among different fluid types with just a click of the mouse. No further adjustment is required for you to be productive immediately. These developments combine the latest discoveries in wavelength, power and timing requirements for ADE with real-time fluid measurement at multiple points during a run. The resulting algorithms solve some of the most significant liquid handling challenges with volatile, low-surface tension and/or meniscus-shifting fluids. Volumetric accuracy and precision for all fluids tested so far—including buffers, nucleic acid and protein solutions, surfactants, serum and cell culture media—are comparable to established performance with DMSO. The Echo® 550 liquid handler introduced the world to acoustic droplet ejection (ADE). This award-winning instrument remains the proven choice for low-volume liquid handling in leading pharmaceutical and biotechnology companies worldwide. Like the Echo 520 and Echo 555 liquid handlers, the Echo 550 uses "touchless" ADE transfer technology. ADE uses sound energy to transfer 2.5 nL droplets from Echo-qualified 384- and 1536-well source plates into 96-, 384-, 1536- and 3456-well destination plates. This unique touchless technology eliminates consumable tip costs, washing, and cross-contamination. Unsurpassed volumetric precision (≤ 8% CV) and accuracy (≤ 10% CV) ensure superior results. Independent studies have shown that Echo liquid handlers eliminate false negatives due to sample loss on tip surfaces in low-concentration transfers. Like the Echo 555 liquid handler, the Echo 550 liquid handler reports both fluid volume and DMSO hydration. Applications: Compound management Compound screening siRNA screening Biochemical assays Cell-based assays PCR Genotyping Gene expression
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Echo® 520 Liquid Handler
Beckman Coulter Life Sciences
Imagine preparing assay plates for siRNA screening, compound screening with biochemical and cell-based assays, PCR reactions and more all on a single liquid handling platform, with no tips and no cross-contamination. Labcyte has developed new acoustic droplet ejection (ADE) parameters that deliver built-in intelligence to enable users to transfer many different fluid types from a single instrument. All Echo systems come pre-calibrated so that you can switch among different fluid types with just a click of the mouse. No further adjustment is required for you to be productive immediately. These developments combine the latest discoveries in wavelength, power and timing requirements for ADE with real-time fluid measurement at multiple points during a run. The resulting algorithms solve some of the most significant liquid handling challenges with volatile, low-surface tension and/or meniscus-shifting fluids. Volumetric accuracy and precision for all fluids tested so far—including buffers, nucleic acid and protein solutions, surfactants, serum and cell culture media—are comparable to established performance with DMSO. The Echo® 520 liquid handler delivers the same superior precision and accuracy as the award-winning 550 and 555 models, for laboratories with lower throughput needs. Like the Echo 550 and Echo 555 liquid handlers, the Echo 520 uses "touchless" ADE transfer technology. ADE uses sound energy to transfer 2.5 nL droplets from Echo-qualified 384- and 1536-well source plates into 96-, 384-, 1536- and 3456-well destination plates. This unique touchless technology eliminates consumable tip costs, washing, and cross-contamination. Unsurpassed volumetric precision (≤ 8% CV) and accuracy (≤ 10% CV) ensure superior results. Independent studies have shown that Echo liquid handlers eliminate false negatives due to sample loss on tip surfaces in low-concentration transfers. The Echo 520 liquid handler reports source well volume, but does not report DMSO hydration values. Applications: • Compound management • Compound screening • siRNA screening • Biochemical assays • Cell-based assays • PCR • Genotyping • Gene expression
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Automated Liquid HandlingAnimal ModelsSample PreparationAcoustic Liquid Handling PlasmaLiquid HandlingLC-MS

Echo® 550 Liquid Handler

Beckman Coulter Life Sciences

Imagine preparing assay plates for siRNA screening, compound screening with biochemical and cell-based assays, PCR reactions and more all on a single liquid handling platform, with no tips and no cross-contamination. Labcyte has developed new acoustic droplet ejection (ADE) parameters that deliver built-in intelligence to enable users to transfer many different fluid types from a single instrument. All Echo systems come pre-calibrated so that you can switch among different fluid types with just a click of the mouse. No further adjustment is required for you to be productive immediately. These developments combine the latest discoveries in wavelength, power and timing requirements for ADE with real-time fluid measurement at multiple points during a run. The resulting algorithms solve some of the most significant liquid handling challenges with volatile, low-surface tension and/or meniscus-shifting fluids. Volumetric accuracy and precision for all fluids tested so far—including buffers, nucleic acid and protein solutions, surfactants, serum and cell culture media—are comparable to established performance with DMSO. The Echo® 550 liquid handler introduced the world to acoustic droplet ejection (ADE). This award-winning instrument remains the proven choice for low-volume liquid handling in leading pharmaceutical and biotechnology companies worldwide. Like the Echo 520 and Echo 555 liquid handlers, the Echo 550 uses "touchless" ADE transfer technology. ADE uses sound energy to transfer 2.5 nL droplets from Echo-qualified 384- and 1536-well source plates into 96-, 384-, 1536- and 3456-well destination plates. This unique touchless technology eliminates consumable tip costs, washing, and cross-contamination. Unsurpassed volumetric precision (≤ 8% CV) and accuracy (≤ 10% CV) ensure superior results. Independent studies have shown that Echo liquid handlers eliminate false negatives due to sample loss on tip surfaces in low-concentration transfers. Like the Echo 555 liquid handler, the Echo 550 liquid handler reports both fluid volume and DMSO hydration. Applications: Compound management Compound screening siRNA screening Biochemical assays Cell-based assays PCR Genotyping Gene expression

(22)

Preparation of Mouse Plasma Microsamples for LC-MS/MS Analysis Using the Echo Liquid Handler

Related products

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Echo® 500 Series Liquid Handlers

Echo® 555 Liquid Handler

Echo® 550 Liquid Handler

Echo® 520 Liquid Handler

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