Preclinical CAR-T cell target safety, biodistribution, and tumor infiltration analysis using the RNAscope ISH assay

16 Mar 2020

In this application note, Advanced Cell Diagnostics explains how RNAscope in situ hybridization (ISH) technology can be used to detect activated CAR-T cells and the low expression of the CAR target antigen.

RNAscope™ Chromogenic Assays

Advanced Cell Diagnostics

Independent of your genes of interest, our easy RNA in situ hybridization assay uses common reagents and protocols; providing universal assay conditions. All RNA-ISH kits contain reagents in a convenient ready-to-use (RTU) format, and chromogenic dyes are available to interrogate a single or two genes simultaneously.

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Preclinical CAR-T cell target safety, biodistribution, and tumor infiltration analysis using the RNAscope ISH assay