Precise spatial multiplexing of protein biomarkers for immune profiling in tissue samples with ChipCytometry

Immunohistochemistry is the most widely used diagnostic technique in tissue pathology. However, IHC is associated with several limitations including the labeling of just a few markers per tissue section and limited quantification of cell populations. As a result of plex limitations, key insights about tumor biology are missed, which could be important for advancing our understanding of tumor biology and ultimately improving patient outcomes. ChipCytometry^™ is a novel image-based platform for precise spatial multiplexing that addresses these challenges by combining iterative immuno-fluorescent staining with high dynamic range imaging to facilitate quantitative phenotyping with single-cell resolution.

In this research poster, Canopy Biosciences demonstrates how standard flow cytometry standard (FCS) files are generated from multichannel OME-TIFF images, enabling the identification of cellular phenotypes via flow cytometry-like hierarchical gating. Quantification of results reveals precise expression levels for each marker in the assay in each individual cell in the sample, while maintaining spatial information about each cell.