ResourceDrug discovery > Basic Research
Ordering information and troubleshooting tips for NGS
4 Jul 2023In this application note, Oxford Gene Technology (OGT) provides ordering information and troubleshooting tips for next-generation sequencing. The note covers various sample quality control issues such as protein contamination, RNA contamination, salt or solvent contamination, and unexpected sample concentration. It also addresses sequencing data quality problems including low coverage uniformity, low on-target coverage, high duplication rate, and low mean target coverage. Additionally, it provides solutions for fragmentation issues and post-capture and pre-capture challenges. Further information and contact details are provided at the end of the note.
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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Quality ControlQuality control is needed in all production processes. Quality control is a monitoring procedure or set of procedures that are put in place to ensure that a manufactured product adheres to a defined set of quality criteria. Contamination PreventionContamination prevention aims to control and minimize the risk of contamination during experiments. Biological safety cabinets and sterilization equipment are examples of technologies used to prevent contamination.Clinical NGSNext Generation Sequencing (NGS) refers to the use of massive parallel sequencing of multiple small fragments of DNA. This high-throughput genomic analysis yields enormous amounts of sequence data, which if appropriately analyzed could have huge potential for clinical laboratories. For this to happen there are technique and bioinformatic hurdles to be overcome.