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NucleoCounter® NC-3000™: Cell Count & Viability of Mammalian Cells using the Via1-Cassette™

ChemoMetec
12 Jul 2021

The NucleoCounter® NC-3000™ system enables the user to perform automated cell counting and analyses of a broad range of mammalian cells. This application note demonstrates how the Via1-Cassette™ used together with the NucleoCounter® NC-3000™ facilitates determination of viability and concentration of cell suspensions by measuring cell counts (total and non-viable) per volume in one simple step.

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NucleoCounter® NC-3000™ advanced cell analyzer

ChemoMetec

The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NC-3000™ can perform high-speed cell count and viability analyses and it includes plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay, a selection of apoptosis assays and a GFP transfection efficiency assay.

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cellular PathologyCellular Pathology deals with the microscopic analysis of tissue samples and cells. Sample preparation and processing includes fixation, staining, sectioning and slide mounting, using equipment such microtomes and cryostats. In choosing immunohistochemistry and immunocytochemistry kits, consider chromogens, staining method, antibodies, microscopes and imaging.Cell ViabilityCell viability assays assess the health of cells and their ability to proliferate. These tests are crucial in drug development, toxicity testing, and cell-based research. Common methods for assessing viability include MTT, live/dead assays, and flow cytometry. Browse our peer-reviewed product directory to find the best tools for cell viability assays, compare products, check reviews, and get pricing directly from manufacturers.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.CytometryCytometry is a broad term for the quantitative analysis of cells and cell systems. The most popular methods to study cytometry are flow cytometry and image cytometry.Cell AnalysisThe analysis of cells allows researchers to understand the factors which contribute to cell health and function. These influencing processes can then be predicted and altered, leading to the development of medication and disease treatments.

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NucleoCounter® NC-3000™: Cell Count & Viability of Mammalian Cells using the Via1-Cassette™