NucleoCounter® NC-3000™ advanced cell analyzer
The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NC-3000™ can perform high-speed cell count and viability analyses and it includes plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay, a selection of apoptosis assays and a GFP transfection efficiency assay.
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Easy to use equipment for routine cell count
Research
Very nice equipment, easy to use, fast to measure 8 samples in a go.
Review Date: 10 Oct 2024 | ChemoMetec
An indispensable device in the laboratory!
general laboratory work
We have been using the NC-3000 (Chemometec) for several years and use it daily in the lab. The system is very versatile as it can not only perform cell counts (including cell cycle and Annexin V assays). In many projects, we work with external partners who need the analyses, for example, for certification of their devices. This is easily possible with the NC3000, which is compatible with 21 CFR Part 11/GMP guidelines.
Review Date: 24 Oct 2023 | ChemoMetec
Easy to use, fast results, consistency in data produced
Day to day cell culture counting; cell numbers & viability
Pros: Fast & easy cell counting, good assay/instrument for keeping track of suspension cell culture growth (when to split culture and numbers to seed for transfection (VPC2.0 from Thermo-Fisher)) We have standardized when to dilute cell culture (expected cell dens above 2E6/ml always dill 1:10; 50µl ad 500µl) Cons: Sometimes (rare events) counting fails due to camera not able to take pictures? Solution is to close program and restart (but you often "loose" a few Via1 cassettes) Regarding software; would be nice to have the time in data set
Review Date: 24 May 2023 | ChemoMetec
The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NC-3000™ can perform high-speed cell count and viability analyses and it includes plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay, a selection of apoptosis assays and a GFP transfection efficiency assay. With the FlexiCyte™ license, you can create protocols choosing light source, emission filters and detailed settings. The accompanying NucleoView™ software can be used for data analysis of the results and has unlimited licenses.
The NucleoCounter® NC-3000™ benchtop image cytometer ensures flow-like analysis with high reproducibility, and it is calibration-free. The NucleoCounter® NC-3000™ is ideal for the regulated QC, production and even PD and R&D departments. Instrument and method verification tools and 21 CFR Part 11-ready software ensures that the adheres to the requirements and demands of the pharmaceutical and biotech industries. Standardized pdf-reports, user control and registration of audit trails allow for documentation of all procedures. Combined with high precision and limited user influence this makes the NucleoCounter® NC-3000™ a one-of-a-kind cytometer.
Our Field Application Scientists (FASs) provide training, service visits, installation and user guidance on-site or online to ensure you get the best start to working with the NucleoCounter® NC-3000™.
Key features:
- No rinsing or clogging
- Easy to operate – no warm-up or calibration
- CFR 21 Part 11 ready
- Automated PDF reports
- Unlimited software licenses and free software updates
- Excellent support by field application specialists
- High-speed cell counting and advanced cell analysis
- Visual inspection of cells – plot to image
- Images are saved automatically and can be re-analyzed later
- Low sample volume
- Build your own assays with up to 4 analytical channels
- Export data to FCS Express, FlowJo, etc.
Quantitative assessment of apoptotic events using image and flow cytometry
Cell death by apoptosis is a complex, tightly regulated process in which a cell orchestrates its own destruction in response to a specific internal or external stimuli. Dysregulation of apoptosis can lead to various physical disorders such as cancer and autoimmunity.
In this scientific poster, ChemoMetec demonstrates how the NucleoCounter® NC-3000™ image cytometer can be used to quantify different events in the apoptosis process.
A comparison of DNA quantitation by image and flow cytometry
In this scientific poster, researchers demonstrate accurate and precise determination of cell cycle stages compared to flow cytometric analyses, by utilizing the NucleoCounter® NC-3000™ from ChemoMetec.
NucleoCounter: Publications review
In 2024, ChemoMetec was featured in over 150 publications spanning various research areas, including cell biology, neurology, gene therapy, oncology, stem cells, bioprocessing, and cell-based immunotherapy.
In this eBook, ChemoMetec presents 30 selected publications that show case the versatile usage of its NucleoCounter® NC-200™, NC-202™, and NC-3000™ instruments across multiple disciplines.
A simple and rapid method for DNA content measurement
DNA content can be determined using fluorescent DNA-selective strains that exhibit emission signals proportional to DNA mass. Cellular fluorescence is measured by flow, image, or laser scanning cytometry.
In this scientific poster, ChemoMetec presents a simple, rapid and robust method for analyzing and quantifying the DNA content of cells, utilizing the NucleoCounter® NC-3000™ image cytometer.
A novel rapid apoptosis assay based on thiol redox status
Apoptosis is a highly regulated process characterized by a number of distinct biochemical and morphological changes.
In this scientific poster, ChemoMetec presents an assay for determining the level and distribution of thiols in a cell population, utilizing the NucleoCounter® NC-3000™ image cytometer.
A decrease in cellular thiols caused by drug-induced apoptosis is easily quantified using the novel vitality assay
In this scientific poster, ChemoMetec presents an assay for determining the level and distribution of thiols in a cell population, utilizing the NucleoCounter® NC-3000™ image cytometer. Additionally demonstrating that a lowered level of cellular thiols correlates with known apoptosis assays.
ChemoMetec achieves gold status with a new Seal of Quality award
ChemoMetec's family of cell counters are being recognized for receiving consistently outstanding customer feedback, again
ChemoMetec achieves silver status, with a new Seal of Quality award
These cell counters are being recognized for receiving consistently outstanding customer feedback, again
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NucleoCounter® NC-200™ automated cell counter
ChemoMetecThe NucleoCounter® NC-200™ is a high precision automated cell counter utilizing state-of-the-art image cytometry. With user-adaptable protocols and specialized assays for counting a wide range of mammalian cells, including adipose-derived stem cells, T cells, aggregated cells and cells on microcarriers, the NucleoCounter® NC-200™ provides an all-in-one solution for cell counting and cell viability determinations.
NucleoCounter® NC-202™ automated cell counter
ChemoMetecThe NucleoCounter® NC-202™ automated cell counter is our 3rd generation cassette-based image cytometer. Featuring improved hardware and new NC-View™ software, we take precision, speed and data management to new levels.
NucleoCounter® Family of Cell Counters and Analyzers
ChemoMetecNucleoCounter® instruments provide platforms for determination of viability and cell count as well as advanced image analysis of yeast, veterinary semen, insect cells and mammalian cells, both primary cells and cell cultures, including cells in aggregate or microcarrier cultures.