ResourceLife Sciences
Ni-NTA Biosensor Quantitation Assays
14 Nov 2017The Ni-NTA biosensor is pre-immobilized with novel nickel-charged tris-nitrilotriacetic (Tris-NTA) groups for quick and easy capture of HIS-tagged molecules. In conjunction with Biolayer interferometry instruments such as the Octet® or BLitz™ systems, the Ni-NTA biosensor provides a rapid and label-free method for HIS-tagged protein quantitation and kinetic analysis.
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BiosensorsBiosensors are devices used to detect an analyte using biological molecules specific to the analyte coupled to a detector. Biosensor instruments may be photometric, typically using surface plasma resonance (SPR), electrochemical or QCM (quartz crystal microbalance) biosensors. Biosensors should be selective, portable, robust and sensitive and have a fast response time. Systems may be manual or automated and usually have associated software.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Label-Free AnalysisProtein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.