Multi-Mycotoxin Screening and Quantitation Using UHPLC, High Resolution and Accurate Mass

28 Jul 2013

In this poster, read about a method for analyzing 32 mycotoxins in beer. The multi-mycotoxyin assay is based on very simple sample preparation and ultra high performance liquid chromatography coupled with full spectral Orbitrap™ MS detection.

Thermo Scientific Exactive Plus Orbitrap LC-MS

Thermo Fisher Scientific

The Exactive Plus Orbitrap LC-MS is a benchtop full scan mass spectrometer designed for compound identification and high-throughput, high-performance screening. The Exactive Plus delivers high resolution accurate mass (HRAM) data and fast, reproducible results for qualitative and quantitative analysis. The availability of HRAM and full scan capability gives you all the data, all the time. It allows retrospective data analysis and eliminates MS method development leading to improved and simplified analytical throughput.Exactive Plus is easy-to-use, cost effective to operate and coupled with workflow-driven software, makes an ideal instrument for new users in routine analytical laboratories.Selected benefits of the Exactive Plus are specified below: High Resolving Power (up to 140,000) - Eliminates isobaric interferences to increase confidence for analysis or complex samples Mass accuracy - Better than 1ppm in full scan and AIF scan using fully automated AGC and mass calibration procedures offers confident identification U-HPLC Scan Speed - The 12Hz scanning frequency of Exactive Plus is fully compatible with U-HPLC and ensures exact mass measurement for fast chromatography applications Fast Polarity Switching - A full cycle of 1 positive and 1 negative scan is performed within 1 second at a resolution setting of 35,000 without sacrificing mass accuracy All-Ion-Fragmentation (AIF) - With multiple dissociation techniques; in source collisional induced dissociation (CID) and high energy collisional dissociation (HCD), provides MS and MS/MS data Full MS scan, AIF scan, data dependent AIF, alternating Full MS and AIF scan Intuitive software interface The Exactive Plus Orbitrap LC-MS is ideal for the following applications: Environmental and Food Safety Clinical Research Forensic Toxicology Drug discovery, metabolism and pharmacokinetics (DMPK) Metabolomics Pharmaceutical BioPharma – Intact Protein analysis Laboratories analyzing food, pharmaceuticals, neutraceuticals, and other complex samples can now purchase Exactive Plus LC-MS system equipped from the factory with IonSense ID-CUBE™ ion sources. This configuration is designed to minimize the need for sample preparation to simplify and speed analyses.

(20)

Links

Tags

UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Food and Beverage AnalyzersFood and Beverage Analyzers are commonly used to provide information about the chemical composition, structure and physical properties of food materials.MycotoxinsToxic chemicals such as Aflatoxin and Alkaloids that are produced by fungi are known as mycotoxins. In humid conditions, fungi can proliferate and colonize on crops to levels that can be damaging to human health. Beer
Multi-Mycotoxin Screening and Quantitation Using UHPLC, High Resolution and Accurate Mass