Monitoring intracellular calcium using fluorescent dyes in a mid-throughput assay

Fluorescent dyes have long been used to monitor intracellular Ca²⁺ levels in living cells by fluorescent imaging. In this application note, BMG Labtech have adapted such protocols for use in a 96-well plate format in the CLARIOstar^® plate reader. Here, Cal-520AM fluorescent dye is shown to have minimal leakage which makes it useful for mid/high-throughput analysis of intracellular Ca²⁺ levels in living cells.