Measurement of SiR-DNA phototoxicity

18 Oct 2022

In this application note, Phasefocus presents a study investigating the effects of far-red fluorescent labeling and LED irradiation on cell behavior during long-term time-lapse microscopy using a Livecyte Kinetic Cytometer.

Livecyte® Cell Analysis System

Phasefocus

Livecyte is a live cell analysis system combining exceptionally high contrast time-lapse videos with automated analysis to provide a complete live cell assay solution.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.CytometryCytometry is a broad term for the quantitative analysis of cells and cell systems. The most popular methods to study cytometry are flow cytometry and image cytometry.Live Cell ImagingLive cell imaging is the study of living cells using microscopes and high-content imaging systems. This technique provides in-depth insight into fast and complex biological processes, by allowing dynamic imaging of living cells instead of acquiring an individual image at a single point in time.
Measurement of SiR-DNA phototoxicity