Low-volume, High Throughput Workflow for Analysis of Nucleic Acid Samples for Biobanking

Accurate quantification of nucleic acids remains a critical step prior to performing downstream processes such as sequencing, mutagenesis, and qPCR. The most common methods of analysis are fluorometric and spectrophotometric methods where the former is used for applications requiring high sensitivity; the latter for quantification of purity and yield from nucleic acid extraction procedures resulting in larger volumes and samples of higher concentration. Quantification is commonly performed in a cuvette or microplate based format. Increasingly, there has been interest in sample conservation and higher throughput analyses more amenable to a microplate format given the ability to analyze smaller volumes, increased sample capabilities, and incorporate automation. This application note demonstrates a higher throughput workflow for the analysis of biobanking samples.