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Kinetic degradation of IMiD molecular glues and their target families

Agilent Technologies
23 Feb 2023

In this application note, Agilent outlines the automation of parallel degradation and viability screening of several key immunomodulatory drug (IMiD) molecular glue targets, IKZF1 (Ikaros), GSPT1, and IKZF2 (Helios) with various IMiDs using the Promega Nano-Glo® HiBiT technology, in conjunction with the Agilent BioTek BioSpa 8 automated incubator and the Agilent BioTek Synergy Neo2 hybrid multimode reader. Targeted protein degradation of disease-causing proteins has proven to be a powerful therapeutic approach. The ability to monitor the specificity of degradation amongst related target classes at endogenous protein levels, continuously in live cells, is critical for understanding the efficacy and mechanism of these compounds.

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Agilent BioTek Synergy Neo2 Hybrid Multimode Reader

Agilent Technologies

Agilent BioTek Synergy Neo2 multimode microplate reader features proprietary Hybrid Technology, with independent optical paths that ensure excellent performance in all detection modes. Variable bandwidth quad monochromators, sensitive high transmission filter-based optics, laser TRF, and up to 4 PMTs provide ultrafast measurements.

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.IncubatorsLaboratory incubators are used to provide a stable environment to cultivate and store samples in, giving you optimal control of temperature, humidity, oxygen and CO<sub>2</sub> levels. Incubators are necessary equipment for cell culture and microbial research. Control temperature with heated or refrigerated incubators, or the levels of CO<sub>2</sub> and oxygen with CO<sub>2</sub> incubators. Using environmental chambers, create a humid climate for testing samples. Find the best incubators in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.Cell IncubationCell incubation involves maintaining controlled environmental conditions for growing and nurturing cells. It is essential for cell culture studies, drug testing, and regenerative medicine. Explore cell incubation solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Cooled IncubatorsProtein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

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