Introducing the RNAscope Plus small RNA-RNA fluorescent assay

In this application note, Advanced Cell Diagnostics introduces the RNAscope™ Plus smRNA-RNA fluorescent assay, a sensitive technology for detecting small regulatory RNA alongside target RNA in intact cells/tissues. This assay can visualize one smRNA target and three RNA targets simultaneously and employs four independent signal amplification systems using different fluorophores. It has a two-week turnaround time for custom designs with over 42,000 pre-designed probes available. The assay has demonstrated detection of positive control miRNA and RNA targets in HeLa cells and mouse colon FFPE tissue, tissue-specific detection of miR-124 in mouse brain tissue, and colocalization of miRNA and associated mRNA targets in head and neck cancer tissue.