RNAscope Plus smRNA-RNA assay
The RNAscope Plus™ smRNA-RNA assay allows simultaneous detection of 1 smRNA and up to 3 RNA targets within a single sample using up to four target probes.
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RNAscope Plus™ smRNA-RNA assay is a valuable new tool to quantify changes in gene expression and cellular function in response to the introduction of regulatory RNAs, essential for optimizing therapeutic efficacy and safety. The RNAscope Plus smRNA-RNA assay allows simultaneous detection of 1 smRNA and up to 3 RNA targets within a single sample using up to four target probes. The assay is compatible with formalin-fixed, paraffin-embedded (FFPE), and fresh frozen (FF) tissue.
Introducing the RNAscope Plus small RNA-RNA fluorescent assay
In this application note, Advanced Cell Diagnostics introduces the RNAscope™ Plus smRNA-RNA fluorescent assay, a sensitive technology for detecting small regulatory RNA alongside target RNA in intact cells/tissues. This assay can visualize one smRNA target and three RNA targets simultaneously and employs four independent signal amplification systems using different fluorophores. It has a two-week turnaround time for custom designs with over 42,000 pre-designed probes available. The assay has demonstrated detection of positive control miRNA and RNA targets in HeLa cells and mouse colon FFPE tissue, tissue-specific detection of miR-124 in mouse brain tissue, and colocalization of miRNA and associated mRNA targets in head and neck cancer tissue.
Assessing safety, efficacy, and biodistribution of small RNAs with RNAscope Plus small RNA-RNA co-detection assay
Advanced Cell Diagnostics demonstrates in this application note how small RNA therapeutics (antisense oligonucleotides and small interfering RNAs) can be visualized and evaluated for their efficacy and safety using RNAscope™ Plus small RNA-RNA fluorescent assay. This assay can visualize small RNAs and RNA targets in various diseases and can assess delivery, biodistribution, uptake, persistence, and efficacy. The RNAscope Plus assay is compatible with TSA Vivid™ dyes and can be automated on the Leica BOND RX autostainer for increased signal intensity.
