Interrogating Gynecological Cancer Cell Metabolisms at Different Oxygen Tensions: Results Using a Novel Modified Atmosphere Handling System

29 Mar 2015

This poster describes a method for the metabolic profiling of gynecological cancer cell lines using a Seahorse Bioscience XFe96 Extracellular Flux Analyzer and a novel workstation that enables analysis to be performed under hypoxic conditions as well as air.

Seahorse XFe24 Analyzers

Agilent Technologies

XFe Extracellular Flux Analyzers simultaneously interrogate the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a microplate, in real-time.

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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Ovarian CancerCancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.HypoxiaCervical Cancer
Interrogating Gynecological Cancer Cell Metabolisms at Different Oxygen Tensions: Results Using a Novel Modified Atmosphere Handling System