SelectScience
Join Free
Accelerating Science
ResourceSpectroscopy

Improving SWATH by Overlapped Windows

SCIEX
12 Jan 2016

This application note investigates the use of overlapping SWATH windows to improve the cycle time and reduce the SWATH window size. The ability to acquire all possible MS/MS fragments during each cycle of data acquisition has radically changed peptide quantitation capabilities. Since no prior information is required, data acquisition is greatly simplified. During data processing, the particulars of which peptides and proteins are studied can be changed at any time, without the need for reacquiring any data. This investigation demonstrated that overlapping windows enabled deconvolution to narrower windows without loss in duty cycle and that narrower windows improved MS/MS quality and quantitative properties.

Related products

Request Quote for All Products

Prominence UFLC

The Prominence UFLC Series is an ultra-high-speed LC that achieves both ultra-high-speed analysis and ultra-high separation, based on high analysis precision and reliability. In addition to shortening analysis times, thereby heightening analysis efficiency and conserving solvent, this instrument supports reliable separation and detection of trace materials in a variety of fields. Applications include the evaluation of trace residual agricultural chemicals to ensure the safety of foods, and the evaluation of trace impurities to further improve product quality in the areas of pharmacology and chemistry. This new LC boasts excellent basic performance and data quality, as well as attention to analysis system versatility and operability, delivering the optimal benefits to a range of laboratory situations. The times call for ultra-high-speed LC. Adopting ultra-high-speed LC for a range of analysis work can provide a variety of benefits. However, it is not enough to simply reduce analysis times. It is also important to maintain basic precision in LC analysis, including retention time repeatability, injection repeatability via an autosampler, and carryover and detector sensitivity. Furthermore, in selecting equipment, high-speed analysis means little without system durability and operability. The Prominence UFLC is the optimal system for any laboratory striving for truly high-throughput analysis. Prominence UFLC Features: Reduces analysis time to 1/10 of conventional LC while keeping separation efficiency. Fast autosampler (10 seconds for 10uL injection), which contributes to a truly fast analysis cycle. Automatic purging, shortens the time required for system start-up via manual operation. Superb high speed and resolution, without applying high pressure, by optimizing system and column efficiency. Proprietary high-precision 6-port valve, which achieves 0.3% or less (RSD) area repeatability in 100,000 cycle Maximizing Data Reliability (5 µL injection, n=6) endurance tests.

(11)

Links

SCIEXCompany website

Tags

Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Software PlatformsSoftware platforms are useful for various stages of laboratory experiments from data collection to data storage and processing. For instance lab software is available for system control, data management, data analysis and qualification / validation.MetabolitesMetabolites are the intermediate and end products of metabolism. Analyzing metabolites offers insight into cellular processes and disease mechanisms and is critical in fields like pharmacology, toxicology, and diagnostics. Explore our peer-reviewed product directory to find the best tools for metabolite analysis, compare products, check reviews, and get pricing directly from manufacturers.Molecular BiologyMolecular biology is the branch of biology that focuses on the molecular mechanisms that underlie cellular functions. It involves studying DNA, RNA, and proteins to understand gene expression, replication, and regulation. Molecular biology is fundamental to biotechnology, medicine, and genetic research. Explore molecular biology products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Cancer DiagnosticsThere are a wide variety of diagnostic tests for cancer available, and this range continues to expand as our knowledge of cancer improves. Current diagnostic methods include biopsy, imaging and blood tests for known biomarkers. New methods in research development include liquid biopsies and cancer breathalyzers.Protein BiologyCompound IdentificationData AnalysisThe analysis of data is the process of transforming, modeling and evaluating data to discover useful information from experimental results. Cancer ResearchCancer research aims to understand the mechanisms of cancer development and progression to improve prevention, diagnosis, and treatment. From molecular biology to clinical trials, research spans a wide range of disciplines, including immunotherapy, targeted therapies, and drug discovery. Explore the best cancer research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Peptide MappingProteomicsProteomics refers to the analysis of proteins through a variety of different experiments in order to understand structure and functionality. Typical techniques include protein purification and mass spectrometry. Data AcquisitionData acquisition refers to the collection and organization of information and data sets.

Related resources