Improving orthopedic diagnostics: The impact of human DNA depletion combined with 16S/18S PCR and sequencing

Identification of the causative agent(s) in bone and joint infections (BJIs) is a crucial but important challenge. Rapid pathogen identification and targeted treatment are critical to prevent serious complications such as bone destruction, joint damage, and sepsis. A considerable number of BJIs are culture-negative or mistakenly identified as aseptic failures, even when diagnostic culture techniques are correctly performed. Molzym presents a diagnostic workflow for the culture-independent detection and identification of bacteria and fungi directly from samples. This workflow offers significant value for the analysis of culture-negative orthopedic samples.