High Throughput Screening of Compounds Affecting Distinct Phases of the Cell Cycle Using Mix-and-Read Assays

In this application note, it is demonstrated how the MultiCyt Cell Cycle Screening Kit and the iQue^® Screener can be employed for high throughput assays that accurately determine cell cycle perturbations across of wide variety of known cell cycle inhibitors. These compounds were selected for their diverse mechanisms of action, including DNA polymerase inhibitors, topoisomerase inhibitors, and compounds that alter microtubule structure. Using both a hand-picked set of eight compounds and a larger, randomly selected 320-compound subset of the NCI Mechanistic Diversity Set library, strong cell cycle blocks in G0/G1, S, and G2/M phases were observed.