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High-Speed Process Monitoring with UHP-SEC-MALS
13 Feb 2017Size-exclusion chromatography (SEC) is a technique for detecting impurities in a biotherapeutic sample that can be coupled with multi-angle light scattering (MALS) to find the molar mass and mass fraction of these species in the sample. Wyatt technology allows this technique to be combined with ultra-high performance (UHP) liquid chromatography to enable high-speed process monitoring. In this application note, UHP-SEC-MALS is used to analyse two antibodies, mAb1 and mAb2, demonstrating how this technique enables rapid monitoring of purification processes.
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microDAWN™
Waters | Wyatt TechnologyThe microDAWN™ determines absolute molecular weight and size of proteins, polymers and other macromolecules in conjunction with UHPLC.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.PurificationPurification techniques are essential for isolating compounds of interest from complex mixtures, such as proteins, nucleic acids, and chemicals. These techniques are critical in biochemistry, drug development, and environmental studies. Explore purification systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Particle Size AnalysisParticle size analysis describes the size distributions from sub-nanometer to millimeter of particulate samples. Size is an important physical property and the technique is often utilized in a range of industries, such as chemical, environmental and agriculture.