ResourceLife Sciences
High-content imaging for diverse 3D cell culture models
23 Sept 20203D cell models are morphologically diverse with varying characteristics based on the cell type and the underlying research questions. In this application compendium, we bring you helpful case studies to perform high-content imaging on a range of 3D models and resolve common challenges experienced in 3D cell culture assays.
Learn how to capture in-depth and high-quality images of spheroids, stem cells, organs-on-chips and whole organisms and read case studies from the scientists developing 3D cell assays to delve into the complex biology of neurodegenerative disease, angiogenesis and tumor microenvironment.
The eBook covers 3D high-content imaging protocols for:
- Characterizing compound effects on 3D cells in extracellular matrix
- Screening cancer therapeutics in spheroids
- Morphological characterization of 3D neuronal networks in an organ-on-a-chip model
- Characterization of angiogenesis in an organ-on-a-chip model
- High-throughput imaging assays using zebrafish
- High-content 3D toxicity assay using iPSC-derived hepatocyte spheroids
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NeuroscienceNeuroscience research investigates the neurological mechanisms underlying behavior, neurodegenerative diseases, and other brain conditions. Learn about the innovative technologies for bioimaging, electrophysiology, cell culture, chromatography and other techniques used in this field.AngiogenesisCell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.NeurobiologyNeurobiology studies the biology of the nervous system, including the structure and function of neurons, glial cells, and their interactions. It is a key field in understanding neurological disorders such as Alzheimer�s, Parkinson�s, and schizophrenia. Research in neurobiology is essential for developing therapies for these conditions. Browse our peer-reviewed product directory to find the best neurobiology research tools, compare products, check reviews, and get pricing directly from manufacturers.Cell AnalysisThe analysis of cells allows researchers to understand the factors which contribute to cell health and function. These influencing processes can then be predicted and altered, leading to the development of medication and disease treatments.Stem Cell ResearchStem cells have the ability to produce identical daughter cells as well as progeny, which commit and differentiate. Stem cell research has exciting prospects of biomedical applications in cellular disease modeling, pharmaceutical screening, and regenerative medicine. 3D Imaging3D imaging technologies allow for the visualization and analysis of three-dimensional structures at high resolution. These systems are used in fields like molecular biology, material science, and medical diagnostics. 3D imaging can be applied to visualize cells, tissues, and organs, providing valuable insights into their structure and function. Browse our peer-reviewed product directory to find the best 3D imaging solutions, compare products, check reviews, and get pricing directly from manufacturers.Live Cell ImagingLive cell imaging is the study of living cells using microscopes and high-content imaging systems. This technique provides in-depth insight into fast and complex biological processes, by allowing dynamic imaging of living cells instead of acquiring an individual image at a single point in time.Cell BiologyCell biology studies the structure, function, and behavior of cells. Understanding cellular processes is essential for research in areas such as cancer biology, stem cell research, and neurobiology. Techniques like flow cytometry, microscopy, and cell culture enable researchers to explore cellular mechanisms in detail. Browse our peer-reviewed product directory to find the best cell biology tools and equipment, compare products, check customer reviews, and get pricing directly from manufacturers.