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FDA Validated Molecular Method to Detect C. cayetanensis in Food Samples

MP Biomedicals
16 May 2019

Download this method to learn how the FastPrep-24™ homogenizer can be used used in combination with the FastDNA™ Spin Kit for Soil and is shown to be an effective method for the lysis of C. cayetanensis oocysts from infected food matrices and isolation of their DNA. The extracted DNA was used successfully in real-time PCR assays that were able to detect as few as 5 oocysts in 25 g of food samples.

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FastDNA SPIN Kit for Soil™

MP Biomedicals

Isolate Pure DNA from Cells Present in Soil or Other Environmental Samples! Thorough lysis in seconds of any microorganism present in environmental samples. Ready-to-use DNA for quantitative and qualitative characterization of microbial soil communities. Excellent reproducibility for an optimum assay-to-assay consistency. Total removal of humic acids and PCR inhibitors for a successful investigation of microbial diversity. The FastDNA™ SPIN Kit for Soil is designed to efficiently isolate bacterial, fungal, plant and animal genomic DNA from soil and environmental samples. Up to 500 mg soil are processed by the FastPrep®-24 5G or and any bead beating homogenizers with the Lysing Matrix E tubes designed to efficiently lyse all microorganisms including difficult sources such as eubacterial spores and endospores, gram positive bacteria and yeast. The released DNA is purified by a silica-based spin filter method and is suitable for PCR and other downstream applications.    

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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.Food SafetyFood safety describes the prevention of food-borne illnesses. Contamination with a variety of pathogens can be prevented with adequate temperature control, hygiene and labeling. Food samples can be tested using GC/MS, HPLC, SPE, and qPCR to ensure their adherence to regulations.Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.Food QualityFood quality refers to the attributes of food that influence its safety, nutrition, and taste. Monitoring and ensuring food quality are essential in production to meet health regulations and consumer expectations. Techniques like spectroscopy, chromatography, and microbial testing are used to assess food properties and detect contaminants. Browse our peer-reviewed product directory to find food quality testing solutions; compare products, check reviews, and get pricing directly from manufacturers.

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FDA Validated Molecular Method to Detect C. cayetanensis in Food Samples