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Fast Turnaround Times and Improved Data Quality for Microbial Whole-Genome Sequencing with a Novel, Single-Tube Enzymatic Fragmentation
Fast Turnaround Times and Improved Data Quality for Microbial Whole-Genome Sequencing with a Novel, Single-Tube Enzymatic Fragmentation
29 Dec 2015Next-generation whole genome sequencing of microbes demands rapid, robust, and scalable library construction workflows, capable of generating high-quality sequence data across a wide range of genome sizes, complexities and genomic GC content. This application note describes a streamlined library preparation method that results in minimal bias, high uniform coverage, and facilitates de novo assembly of microbial genomes.
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KAPA Hyper Prep Kits
Kapa Biosystems, Inc.Versatile, streamlined solution for DNA library preparation for Illumina sequencing.
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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Library GenerationLibrary generation refers to the construction of NGS libraries from RNA and DNA sources.DNA Fragment SizingMicrobial DetectionMicrobial detection is crucial for identifying and quantifying bacteria, viruses, fungi, and other microorganisms. This process is essential for food safety, environmental monitoring, clinical diagnostics, and pharmaceutical manufacturing. Explore the best microbial detection tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Genome EditingGenome editing involves altering the DNA of an organism, which holds promise for disease treatment, agriculture, and gene therapy. Techniques like CRISPR-Cas9 are revolutionizing this field, enabling precise genetic modifications. Explore genome editing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.EnzymesGenome SequencingGenome sequencing involves determining the complete DNA sequence of an organism's genome. It provides crucial information about genetic variations, mutations, and diseases. Advances in sequencing technologies, such as next-generation sequencing (NGS), have accelerated research in genomics, diagnostics, and personalized medicine. Explore genome sequencing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.