KAPA HyperPlus Kits

The KAPA HyperPlus kit provides a streamlined workflow that includes fragmentation and library preparation in a single tube.

Building on industry-leading library construction efficiencies, this integrated solution combines enzymatic fragmentation, similar in quality to mechanical shearing, with the speed and convenience of tagmentation-based workflows.

Benefits:

• DNA fragmentation and library prep in 2.5 hours
• Flexible DNA sample input from 1 ng – 1 µg
• Reduced bias and maximize sequence coverage
• PCR-free workflows

Features:

Tunable and Reproducible Fragmentation

• Adjust library insert sizes from 150 – 800 bp by varying fragmentation time
• Reproducible insert sizes across a range of GC content and DNA input amounts

Industry Leading Library Yields…

• Routinely achieve conversion rates >80% from ≥ 100 ng input
• Superior performance across a range of DNA input amounts
• High library yields enable PCR-free workflows from as little as 50 ng starting material

…Enable Superior Sequencing Results

• Higher conversion rates results in fewer amplification cycles and lower duplication rates
• Detect low-frequency mutations with high confidence due to greater library diversity and more uniform sequence coverage

Minimal Sequence Coverage Bias

• Lower sequence bias when compared to tagmentation and other enzymatic fragmentation methods
• Equivalent performance to mechanical shearing
• Less bias leads to more uniform sequencing coverage and reduced sequencing costs

Applications:

• Whole Genome Sequencing
• Whole Exome Sequencing
• Targeted Sequencing (custom panels)
• FFPE Sample Sequencing