ResourceLife Sciences
Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient
14 Nov 2013This tech report focuses on the use of the thermal gradient feature on the CFX96™ real-time PCR detection system to optimize reverse transcription (RT) and fast PCR conditions for a four-target multiplex PCR assay. It is demonstrated that the gradient feature of the CFX96 system dramatically accelerates the optimization of the RT and PCR steps for multiplexing RT-qPCR assays and improves the ease of converting cycling conditions from a three-step to a two-step PCR assay.
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CFX96 Touch™ Deep Well Real-Time PCR Detection System with Starter Package
Bio-RadModular thermal cycler platform, includes C1000 Touch™ thermal cycler chassis, CFX96 Deep Well™ optical reaction module, CFX Manager™ software, qbasePLUS software license, cables, reagents, consumables
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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.RT qPCRReal-time quantitative PCR (RT-qPCR) is a technique used to measure gene expression and quantify DNA or RNA in real-time. It is essential in genomics, diagnostics, and biotechnology research. Explore RT-qPCR systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Real-Time PCRReal-time PCR (RT-PCR) is a technique used to amplify and simultaneously quantify nucleic acids in real time. It is a key tool in gene expression analysis, pathogen detection, and diagnostics. RT-PCR allows for the detection of specific RNA or DNA sequences with high sensitivity and specificity. Browse our peer-reviewed product directory to find the best Real-Time PCR systems; compare products, check reviews, and get pricing directly from manufacturers.