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Extraction of Labile Enzymes from Gram-Positive Bacteria with the FastPrep System

MP Biomedicals
18 Sept 2014

This application note demonstrates the capacity of the FastPrep™ system using FastProtein Blue matrix to successfully extract unstable enzymes such as Bam HI and Sau 3AI from gram-positive bacteria. The FastPrep™ system can reduce lysing time by approximately 60% compared to sonication methods and efficiency is also improved.

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Lysing Matrices

MP Biomedicals

  Lysing Matrices tubes are plastic tubes containing specific beads designed for homogenization of any sample.   The sample material is disrupted through multidirectional simultaneous impacts with the specialized Lysing matrix beads in a bead beater instrument, usually in presence of a lysis buffer. Although Lysing Matrix have been designed to be used with the FastPrep ® system, the standard size of the tubes allows their use with many other bead beaters. The lysing matrix tubes are available in many sizes, ranging from 2ml to 50ml and 96-well plates. The wide range of Lysing Matrix types ensure optimal homogenization of any sample.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.EnzymesEnzymes are macromolecular biological catalysts used across life science, pharmaceutical and clinical research. Various enzymes can be purchased for research applications including ligases, high fidelity polymerases, nuclease, protease, restriction enzymes, tissue dissociation enzymes and enzyme inhibitors. Find the best enzymes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BacteriaBacteria are microorganisms that play critical roles in the environment, human health, and biotechnology. Understanding bacterial genetics, growth, and metabolism is essential for developing antibiotics, vaccines, and improving industrial processes. Browse our peer-reviewed product directory to find the best bacterial research tools, compare products, check reviews, and get pricing directly from manufacturers.

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