ResourceFood and Beverage
Evaluation of Several Columns and Solvents for Post-Extraction Gel Permeation Chromatography Clean-up of Fish Tissue Prior to PCB Analysis
28 Sept 2017The determination of PCBs, as well as other environmental contaminants, in fish tissue requires extensive sample cleanup prior to analysis by gas chromatography with an electron capture detector (GC-ECD). The high fat content of fish tissue can cause buildup of nonvolatile materials on the GC injection port and the analytical column. This application note evaluates several GPC clean-up columns and mobile phases that would give faster throughput and use less solvent for the post-extraction clean-up of fish tissue prior to PCB analysis via GC-ECD.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. 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