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Evaluation binding of individual and combined domains in the bacterial flagellar motor complex by CG-MALS

Waters | Wyatt Technology
29 Mar 2020

In this application note, Wyatt Technology utilizes composition-gradient multi-angle static light scattering (CG-MALS) to explore the interactions of FliG with FliM, two key proteins involved in the bacterial flagellar motor switching mechanism. This technique, incorporating the Calypso system with the inline DAWN MALS detector, facilitates the confirmation of specific binding, measurement of equilibrium dissociation constants, and the identification of the stoichiometries of formed complexes.

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DAWN™

Waters | Wyatt Technology

The DAWN is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution, offering the highest sensitivity and widest range of molecular weight, size, concentration and optional features.

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