Enabling His-tagged protein analysis in expression workflows
8 Sept 2025His-tagging is a widely used strategy for protein purification, screening, and assay development – offering simple affinity-based capture without altering protein function.
In this application note, Abselion demonstrates Amperia™, offering a practical alternative, enabling quantification of His-tagged proteins even from crude samples using a premix competition assay.
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Amperia™ Protein Quantification System
AbselionThe Amperia™ system combines compact hardware, assay-ready sensors, and integrated software to streamline protein quantification. Built to support scientists in discovery, development, and beyond, it delivers dependable data quickly and without unnecessary complexity — even from crude or complex samples.
Tagged Protein Quantification Kit | His-Tag (SKU: AK-HIS-004)
AbselionThe His-Tag Protein Quantification Kit extends the Amperia™ Protein Quantification System for direct, tag-specific measurement of His-tagged recombinant proteins. Using a premix competition format with GenScript’s THE™ His Tag Monoclonal Antibody, the kit delivers accurate, reproducible results across both crude lysates and purified samples, with up to 80 tests per kit.