Enabling His-tagged protein analysis in expression workflows

8 Sept 2025

His-tagging is a widely used strategy for protein purification, screening, and assay development – offering simple affinity-based capture without altering protein function.

In this application note, Abselion demonstrates Amperia™, offering a practical alternative, enabling quantification of His-tagged proteins even from crude samples using a premix competition assay.

Amperia™ Protein Quantification System

Abselion

The Amperia™ system combines compact hardware, assay-ready sensors, and integrated software to streamline protein quantification. Built to support scientists in discovery, development, and beyond, it delivers dependable data quickly and without unnecessary complexity — even from crude or complex samples.

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Protein ExpressionProtein expression is the utilization of cell machinery for the synthesis of proteins and has become a critical tool in biotherapeutic, genomic, and proteomic research. Produce recombinant proteins with expression vectors in combination with a host cell suitable for high-level protein expression. For production of toxic proteins, consider cell-free expression vectors. Create and monitor post-translational modifications with protein modification kits. Find the best protein expression products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Enabling His-tagged protein analysis in expression workflows