ResourceDrug discovery > Basic Research
Development of a target-capture NGS assay for use in molecular-based research of myeloid measurable residual disease
4 Jul 2023In this poster, Oxford Gene Technology (OGT) highlights the increasing role of molecular methods, specifically next-generation sequencing (NGS), in detecting measurable residual disease (MRD) in conditions like acute myeloid leukemia (AML). While real-time qPCR (RQ-PCR) and digital droplet PCR (ddPCR) are highly sensitive, they have limitations in detecting multiple targets in a single assay. NGS overcomes this limitation by allowing the evaluation of many genes and variant types in a single assay. OGT has developed a target-capture NGS approach to support researchers in studying molecular-based MRD monitoring in myeloid malignancies, offering improved accuracy and cost-effectiveness.
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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LeukemiaqPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.Flow Cytometry