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Developing air-dried, inhibitor-tolerant qPCR assays for point-of-care diagnostics

PCR Biosystems
22 Jul 2024

Air-drying has emerged as a simple, fast, and cost-effective option for developing dried-format qPCR-based diagnostic kits. Assays with better resistance to inhibitors allow for improved diagnostic accuracy, fewer extraction steps, faster results, and ultimately lower testing costs. The Air-Dryable Inhibitor-Tolerant mixes from PCR Biosystems are designed to meet this need. Discover how they combine precise qPCR with broad-spectrum inhibitor tolerance, providing a simple, low-cost route for kit manufacturers to develop dried assay formats.

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Air-Dryable Inhibitor-Tolerant Probe Mix

PCR Biosystems

Air-Dryable Inhibitor-Tolerant Probe Mix is a glycerol-free 4x qPCR mix that combines our novel inhibitor-tolerant chemistry alongside our cutting-edge air-dryable technology. Now you can benefit from dried, room-temperature stable qPCR assays capable of highly-sensitive DNA detection on the most challenging, inhibitor-rich sample types.

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Air-Dryable Inhibitor-Tolerant Probe 1-Step Mix

PCR Biosystems

Air-Dryable Inhibitor-Tolerant Probe 1-Step Mix is a glycerol-free 4x RT-qPCR mix that combines our novel inhibitor-tolerant chemistry alongside our cutting-edge air-dryable technology. Now you can benefit from dried, room temperature stable qPCR assays capable of highly-sensitive RNA & DNA detection on the most challenging, inhibitor-rich sample types.

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Assay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.RT qPCRReal-time quantitative PCR (RT-qPCR) is a technique used to measure gene expression and quantify DNA or RNA in real-time. It is essential in genomics, diagnostics, and biotechnology research. Explore RT-qPCR systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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