Determination of Pesticide Residues in Drinking Water using Automated Solid-Phase Extraction and Gas Chromatography with Nitrogen Phosphorus Detection

14 Sept 2015

Organophosphorous pesticides have been widely used to prevent and treat plant diseases and pests in agricultural products and as a result, excessive residual pesticide content is frequently reported in drinking and surface water sources. A number of organophosphorous pesticides are acutely toxic to human and animal life, making the detection of residual organophosphorous pesticides a growing concern. This application note addresses the detection of trace amounts of organophosphorus pesticides in water and optimizes the conditions for solid-phase extraction (SPE) and chromatographic analysis using the Thermo Scientific™ Dionex™ AutoTrace 280 Solid-Phase Extraction instrument and the Thermo Scientific™ TRACE™ 1300 Series gas chromatograph with a nitrogen-phosphorus detector (NPD).

Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction Instrument

Thermo Fisher Scientific

The Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction (SPE) instrument is a powerful, high-throughput workstation dedicated specifically for automating SPE. The Dionex AutoTrace 280 instrument automates the cartridge or disk conditioning, sample loading, rinsing, and eluting steps for large volume aqueous solution extractions (20 mL to 20 L).The unit can process up to six samples in parallel, automatically conditioning, washing, and eluting SPE cartridges or disks with a choice of up to five reagents. The unit also segregates aqueous and solvent waste using separate waste lines for efficient, cost effective waste handling.AutoTrace 280 Solid-Phase Extraction Instrument Features: Use SPE technology for liquid-liquid extraction Automated sample loading of liquids onto SPE cartridges Automated eluting of SPE cartridges with organic solvent Closed systems with a fan to vent solvent vapors Positive pressure loading and elution of samples and solvents Benefits: Reduced solvent usage and elimination of glassware Does not require technician involvement to maintain a reservoir of liquid in the SPE cartridge Does not require technician involvement to control the flow of organics solvent through the SPE cartridge No hood required Provides constant flow of liquids through SPE cartridges

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Thermo Scientific™ TRACE™ 1300 Series GC System

Thermo Fisher Scientific

The Thermo Scientific TRACE 1300 Series GC offers the most versatile gas chromatograph platform in the market, with unique "instant connect" modularity for ground-breaking ease of use and performance, setting a new era in GC technology.The Thermo Scientific TRACE 1300 Series GC is the ideal budget-conscious investment for the basic routine laboratory when lower operator expertise requires ease of use with minimal instrument interaction. Its simplified user-interface is also ideal for 24/7 operations that require single-button start/stop/maintenance local interactions while maintaining full programmability through the networked control software.Interchangeable modules free your laboratory from the challenges and constraints of the past. Remove the complexity and eliminate the need for specialized service assistance or new system requirements with modular injectors and detectors.Thermo Scientific Trace 1300 Series GC Features: Analyze dirtier matrices with minimum cleanup using the system's robust injector technology Analyze trace concentrations with smaller injection amounts and minimum preconcentration results using enhanced detector sensitivity and micro-volume technology Protect system components and reduce run time and maintenance using optional backflush capabilities Shorten sample cycle times using low thermal mass injectors and detectors and fast oven technology Automate your entire workflow—from pipetting to injection—using a full range of sample handling options Streamline and speed up data analysis using Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data System (CDS) software Expands easily from single-channel to multi-injector/detector GC configurations Reduces training and startup time with simplified installation, plug-in components, minimal local control and intuitive Chromeleon software Minimizes downtime with easy-access, tool-free modules Reduces operational costs by allowing use of standard consumables Saves energy with low thermal mass and quick start-up Minimizes sample and solvent consumption with micro-volume sample capability Modular design lets you easily reconfigure to meet changing requirements

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Gas ChromatographyGas chromatography (GC) is an analytical technique used to separate and quantitate mixtures of small and volatile compounds. Gas chromatographs or GC systems include components such as GC columns, detectors, pumps and autosamplers. Choose from packed or capillary GC columns, flame ionization (FID), photoionization (PID) electron capture detectors and selective or non-selective detectors. Find the best gas chromatographs in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Environmental Monitoring and TestingEnvironmental monitoring and testing uses handheld portable analyzers, kits, spectrometers or chromatography systems for air, water, soil, food and other sample testing. Useful features of analyzers such as BOD and COD include portability, easy calibration, automation and sensitivity.  Environmental test kits for pH, water, moisture, etc, should be accurate, sensitive, reliable, fast and easy to use.Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Food and Beverage AnalyzersFood and Beverage Analyzers are commonly used to provide information about the chemical composition, structure and physical properties of food materials.Drinking Water AnalysisAnalytical methods used to test the purity of drinking water include determining the level of contaminants abides to the level set by the Environmental Protection Agency (EPA).PesticidesPesticides are used to protect crops from diseases, pests and unwanted plants. They are classed as persistent organic pollutants (POPs) and can be harmful to humans. The Environmental Protection Agency (EPA) currently regulates the use of pesticides in the USA. Food SafetyFood safety describes the prevention of food-borne illnesses. Contamination with a variety of pathogens can be prevented with adequate temperature control, hygiene and labeling. Food samples can be tested using GC/MS, HPLC, SPE, and qPCR to ensure their adherence to regulations.Solid Phase Extraction (SPE)Solid phase extraction (SPE) is a sample preparation technique used to separate and concentrate analytes from complex mixtures. It is widely used in environmental, pharmaceutical, and clinical analysis. SPE provides higher sensitivity and selectivity compared to traditional liquid-liquid extraction methods, making it essential for accurate and reproducible results. Explore SPE tools and systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Nitrogen
Determination of Pesticide Residues in Drinking Water using Automated Solid-Phase Extraction and Gas Chromatography with Nitrogen Phosphorus Detection